关键词: 脑栓通胶囊, 脑缺血再灌注, 神经功能, 脑梗死体积, 血管新生

Abstract: Objective To study the effect of Naoshuantong capsule on angiogenesis in rats with focal cerebral ischemia reperfusion. Method Healthy adult male SD rats were induced to develop occlusion of the middle cerebral artery by suture embolus, Then, the rats were randomly divided into the sham group, a model group, and a low-dose Naoshuantong capsule and high-dose Naoshuantong capsule groups ( 1. 05, 2. 62 g / kg, respectively) . Naoshuantong capsule was administered once per day for 7 days, from 24 h after surgery a day. To examine the recovery of neurological function, we measured modified neurological severity score (mNss) method ; the infarction volume was measured by 2, 3, 5-triphenyltetrazolium chloride ( TTC) staining technique; Western blot was used to detect the expression of angiogenesis related proteins in rat cerebral cortex; Angiogenesis was detected by immunofluorescence staining. Result Compared with those in the sham group,the scores of neurological behavior and the cerebral infarction volume ratios in the model group were significantly increased ( P < 0. 001) . Compared with that in the model group,the low and high-dose Naoshuantong capsule groups exhibited a dramatic improvement of the neurological behavior scores ( P < 0. 05 ) , Naoshuantong capsule high-dose groups exhibited a significant reduction in the volume of cerebral infarction ( P < 0. 05 ) . Compared with the model group, the expression of cortical angiogenesis-related proteins vascular endothelial growth factor (VEGF) and basic fibroblast growth factor ( bFGF) in the Naoshuantong capsule low and high-dose groups were significantly increased( VEGF:P< 0. 05,P< 0. 001;bFGF:P < 0. 05,P < 0. 001) ; Compared with the model group, the number of Lectin + / Ki67 + cellls were significantly increased ( P < 0. 05, P < 0. 001 ) . Conclusion Naoshuantong capsule may promote angiogenesis to improve neurological function after ischemia in rats.

Key words: Naoshuantong capsule, cerebral ischemia-reperfusion, neurological function, cerebral infarction volume, angiogenesis